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Optimisation of ammonium sulfate precipitation method …
Int J Anal Bio-Sci Vol. 2, No 1 (2014). Original Article . Optimisation of ammonium sulfate precipitation method to achieve high throughput concentration of crude alkaline phosphatase from Brown shrimp (Metapenaeus monoceros) hepatopancreas Krishna Prasad Nooralabettu Summary Effectiveness of various pH and ammonium sulfate saturation level in the pancreatic samples that has been homogenised at 3,000 rpm for 10 min at 4 and clarified at RCF of . g for 5min at 4 to concentrate alkaline phosphatase with optimum yield and purity was determined. precipitation performed using M Tris-HCl buffer of pH containing M MgCl2 and ZnCl2. produced optimum yield and activity in comparison to the precipitation performed using 2 M KCl solution of pH 7, and hence components and pH of the buffer plays a major role during concentration.
1. Introduction Hepatopancreas of shrimps is an important organ functions in food absorption, transport, secretion of digestive enzymes, and storage of lipids, glycogen, and
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