Transcription of Optimizing the Human Whole Exome Sequencing Process
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Optimizing the Human Whole Exome Sequencing Process
Application NoteAuthorsRaouf Ben Abdelali, Laboratoire CERBA, Saint-Ouen L Aum ne, France J r me Audoux, SeqOne, Montpellier, France Detlef Trost, Laboratoire CERBA, Saint-Ouen L Aum ne, France Anissa Zouaoui, SeqOne, Montpellier, France Aicha Boughalem, Laboratoire CERBA, Saint-Ouen L Aum ne, France Ramdane Mallek, Laboratoire CERBA, Saint-Ouen L Aum ne, FranceAdrien Jeanniard, Agilent Technologies Nicolas Philippe SeqOne, Montpellier, FranceRoubila Meziani, Agilent TechnologiesJean-Marc Costa, Laboratoire CERBA, Saint-Ouen L Aum ne, France SummaryThe Laboratoire Cerba (Cerba) has been working with Agilent and SeqOne to optimize its Whole Exome Sequencing (WES) Process .
Eight genomic DNA samples were tested: one Agilent female reference DNA (part number 5190-8850), one Coriell DNA (NA12878), and six constitutional DNA samples extracted from blood (five with intellectual disability and one fetus with polymalformation). Total blood was collected in EDTA tubes. DNA was
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