Transcription of Part# 9PIM180 Revised 4/18
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Part# 9PIM180 Revised 4/18
Certificate of AnalysisQuality Control AssaysPromega Corporation2800 Woods Hollow RoadMadison, WI 53711-5399 USAT elephone608-274-4330 Toll USE LIMITATIONS, WARRANTY, DISCLAIMERP romega manufactures products for a number ofintended uses. Please refer to the product label for theintended use statements for specific products contain chemicals which may beharmful if misused. Due care should be exercised withall Promega products to prevent direct human Promega product is shipped with documentationstating specifications and other technical products are warranted to meet or exceed thestated specifications.
T4 DNA Ligase (Weiss units) 0.1–1u Nuclease-Free Water to final volume of 10µl 2. Incubate the reaction at: room temperature for 3 hours, or 4°C overnight, or 15°C for 4–18 hours. Notes: 1. There is considerable latitude in the temperature and time needed for successful ligations.
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