Certificate of Analysis GoTaq Part# 9PIM712

Certificate of Analysis GoTaq® Green Master Mix Cat.# Size M7122 100 reactions M7123 1,000 reactions Cat.# M7122 and M7123 include GoTaq® Green Master Mix, 2X, and Nuclease-Free Water. Description: GoTaq® Green Master Mix(a) is a premixed ready-to-use solution containing bacterially derived Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal …

  Analysis, Certificate, Certificate of analysis

AMP-Glo Assay Technical Manual TM384 - Promega

The AMP-Glo™ Assay can be performed with different plate formats; the recommendations below show the guideline volume setup per well (Table 1). Volume size between Step 1 and Step 2 also may be changed if you follow the

  Manual, Technical, Technical manual

Temperature Dependence of pH for Commonly Used Buffers.

TECHNICAL REFERENCE PROMEGA CORPORATION 2800 WOODS HOLLOW ROAD MADISON, WI 53711-5399 USA TELEPHONE 608-274-4330 www.promega.com ©2010 ALL RIGHTS RESERVED PART #GE647 Temperature Dependence of pH for Commonly Used Buffers

  Temperatures, Dependence, Temperature dependence of ph for

Part# 9PIM180 Revised 4/18

The ligation conditions given in this protocol are based on the conditions used at Promega for quality control of lambda vectors with sticky ends. These ligation condi-tions have been developed using Promega Blue/White Cloning-Qualified T4 DNA Ligase. 3. The addition of polyethylene glycol (PEG) to ligation reactions can promote ligation of

  Ligation

pGEM -T and pGEM -T Easy Vector Systems - Promega

Rapid Ligation: The pGEM ®-T and pGEM -T Easy Vector Systems are supplied with 2X Rapid Ligation Buffer. Ligation reactions using this buffer may be incubated for 1 hour at room temperature. The incubation period may be extended to increase the number of colonies after transformation. Generally, an overnight incubation at 4°C produces

  Ligation

CellTiter-Glo Luminescent Cell Viability Assay Technical ...

washing, removal of medium or multiple pipetting steps are not required. ... Each vial of substrate is sufficient for 1,000 assays at 100µl/assay in 96-well plates or 4,000 assays at 25µl/assay in 384-well plates (10,000 to 40,000 total assays). Includes:

  Washing, Vial

Dual-Luciferase Reporter Assay System - Promega

Dual reporters are commonly used to improve experimental accuracy. The term “dual reporter” refers to the simultaneous expression and measurement of two individual reporter enzymes within a single system. Typically, the

  Dual

pGL3 Luciferase Reporter Vectors - Promega

NcoI 86 luc+ Synthetic poly(A) signal / transcriptional pause site (for background reduction) SV40 late poly(A) signal (for luc+ reporter) HpaI 1902 Ampr 0745V A08_4A Figure 2. The pGL3-Enhancer Vector circle map. Additional description: luc+, cDNA encoding the modified

  Coins

pGL4 Luciferase Reporter Vectors - Promega

start of the reporter gene (the NcoI restriction site) to the bacterial origin of replication sequence was also redesigned. The modifications to this region include: a greatly reduced number of consensus transcription factor binding sites

  Coins

The Pico DNA Quantitation Using PicoGreen

Final DNA Concentration in PicoGreen Assay 1000 0 1000 1000 ng/mL 500 500 1000 500 ng/mL 100 900 1000 100 ng/mL 10 990 1000 10 ng/mL 1 999 1000 1ng/mL 0 1000 1000 blank Table 1. Protocol for preparing standard curve. 3.2.3 After incubation calibrate the Picofluor Fluorometer. Press “STD VAL” and enter “999” for the highest standard ...

  Final, Pico

Post-Transfection Analysis of Cells - Bio-Rad

Post-Transfection Analysis of Cells Flow Cytometry Fluorometry Laser-Scanning Molecular Imaging Luminometry Microscopy Real-Time Quantitative PCR Spectrophotometry Western Blot Analysis References positive cells within a transfected population

  Analysis, Post, Transfection, Cells, Post transfection analysis of cells

TRANSFECTION PROTOCOL Lipofectamine 3000 …

transfection is acceptable, but replace with complete growth medium within 4–24 hr post-transfection. • Antibiotics can be used during transfection. • Prior to flow cytometry, visualize cells under a bright-field microscope to verify dissociation following incubation with TrypLE reagent. Figure 1. Post-transfection analysis of cells.

  Analysis, Post, Protocol, Transfection, 3000, Cells, Post transfection analysis of cells, Transfection protocol lipofectamine 3000, Lipofectamine

安定型細胞株作成のガイドライン - lonzabio.jp

transfection method and cell type. Plate transfected cells and cultivate cells in medium without G418. Do not add G418 to culture medium immediately after transfection as this may drastically increase mortality. Dilute cells into culture plates and start selection 24 – 48 hours post-transfection. Feed every 2 – 3 days (for batch

  Post, Transfection, Cells

THE J B C © 2000 by The American Society for Biochemistry ...

In some cases, cells were cultured with 100 mM benzoyl-Val-Ala-Asp-fluoromethylketone (Bachem) beginning at 1 h after transfection. For assessing the intracellular location of DBok, transfected cells were lysed by homogenization in hypotonic detergent-free buffer (22) and nuclei were discarded by centrifugation at 500 3 g for 5 min. Post-

  Post, Transfection, Cells

Transfection of 293F Mammalian Cells using PEI

Cell/Freestyle media until the time of transfection. The cells are then collected by centrifugation and resuspended in Freestyle™ 293 Medium alone at a 2.5 x 106 cells/ml. DNA and PEI addition is identical to transfections of Freestyle™ 293-F cells and the cells are maintained in the Freestyle™ 293 medium for 24 hours post-transfection.

  Post, Transfection, Cells

Setting Up a PCR Laboratory - BioSupplyNet

Amplicon Aerosol The single most important source of PCR product contamination is the generation of aerosols of PCR amplicons that is associated with the post-PCR analysis.

  Analysis, Laboratory, Testing, Post, Setting up a pcr laboratory

A simple electroporation method of green fluorescent ...

Fig. 5 A set of macroscopic views of intact lingual tissue and in vitro imaging of the eGFP fluorescence at 24, 48, and 72 h post- transfection in experiment 2. Arrowheads: eGFP-transfected area. Scale bars = 2.0 mm In experiment 3, we configured the number of transfer pulses to be fixed at 30 and adjusted the pulse voltages to be

  Post

Pharmacology, Physiology, and Mechanisms of Incretin ...

Cell Metabolism Review Pharmacology, Physiology, and Mechanisms of Incretin Hormone Action Jonathan E. Campbell 1and Daniel J. Drucker ,* 1Department of Medicine, Samuel LunenfeldResearchInstitute,MountSinai Hospital,Universityof Toronto,Toronto,ON M5G 1X5,Canada *Correspondence: d.drucker@utoronto.ca

  Physiology, Mechanisms, Pharmacology, And mechanisms of

How to measure miRNA expression - University of Bristol

E.g. microRNAs and disease In human cancer, miRNA expression profiles differ between normal tissues and the tumours that are derived from them, and differ between tumour types

  Measure, Expression, Marin, How to measure mirna expression

Novel TCRs for Cancer Therapy - agentustherapeutics.com

Novel TCRs for Cancer Therapy May 2, 2018 A subsidiary of Agenus Inc.