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Transfection Protocol Lipofectamine 3000

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96-well 24-well 6-well - Thermo Fisher Scientific

tools.thermofisher.com

Lipofectamine ™ 3000 Reagent Protocol. Protocol Outline. A. Plate cells so they will be 70–90% confluent at the time of transfection. B. Prepare plasmid DNA-lipid complexes (recommend 2 doses of lipid). C. Add DNA-lipid complexes to cells. Transfection Amounts Transfection of siRNA. To transfect cells with siRNA, follow the protocol as ...

  Protocol, Transfection, 3000, Lipofectamine, Lipofectamine 3000

Protocol: Lipofectamine 3000 Transfection Reagent HepG2 ...

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Transfection protocol Transfection components Component Cat. No. Thermo Scientific ™ Nunc 24-Well Cell-Culture Treated Multidishes 142475 Invitrogen ™ Lipofectamine 3000 Transfection Reagent L3000008 Gibco ™ Opti-MEM I Reduced Serum Medium 31985062 Step Tube Complexation components Amount per well (24-well)

  Protocol, Transfection, 3000, Lipofectamine, Lipofectamine 3000 transfection, Transfection protocol transfection

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