Transcription of Protocol: Lipofectamine 3000 Transfection Reagent HepG2 ...
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Protocol: Lipofectamine 3000 Transfection Reagent HepG2 ...
Transfection protocol Lipofectamine 3000 Transfection ReagentHepG2 Liver cancer cellsComplete growth mediumComponentCat. DMEM with GlutaMAX Supplement1056601610% Gibco FBSA3160401 Proper culture techniques and procedures are an essential part of ensuring successful Transfection . Subculturing, also referred to as passaging, is the removal of medium and transfer of cells from a culture into fresh growth medium, in order to propagate the : HepG2 cells tend to grow in clusters. It is critical to ensure dissociation has occurred to yield a single-cell suspension, when passaging and plating the cells. Having a single-cell suspension is also very important to ensure efficient Maintain cells in T-75 flasks. Use Gibco Tr y p L E dissociation Reagent . Passage cells every 3 4 days to ensure that they do notenter senescence. Transfection of cells should be performed only betweenpassages 5 and 25 post-thaw. If designing an experiment that involves Transfection ,ensure that setup coincides with a cell passage.
Transfection protocol Transfection components Component Cat. No. Thermo Scientific ™ Nunc 24-Well Cell-Culture Treated Multidishes 142475 Invitrogen ™ Lipofectamine 3000 Transfection Reagent L3000008 Gibco ™ Opti-MEM I Reduced Serum Medium 31985062 Step Tube Complexation components Amount per well (24-well)
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