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Analyzing Differences in Bacterial Optical Density ...

Analyzing Differences in Bacterial Optical Density Measurements between SpectrophotometersBrian C. Matlock1, Richard W. Beringer1, David L. Ash1, Michael W. Allen2 and Andrew F. Page11 ThermoFisher Scientific, UV-Vis/Fluor-Molecular Spectroscopy, Wilmington, DE. 2 ThermoFisher Scientific, UV-Vis/Fluor-Molecular Spectroscopy, Madison, Density (OD) measurement of Bacterial cultures is a common technique used in microbiology. Researchers have primarily relied on spectrophotometers to make these measurements, however the measurement is actually based on the amount of light scattered by the culture rather than the amount of light absorbed.

Analyzing Differences in Bacterial Optical Density Measurements between Spectrophotometers Brian C. Matlock 1, Richard W. Beringer 1, David L. Ash , Michael W. Allen2 and Andrew F. Page 1ThermoFisher Scientific, UV-Vis/Fluor-Molecular Spectroscopy, Wilmington, DE. 2ThermoFisher Scientific, UV-Vis/Fluor-Molecular Spectroscopy, Madison, WI. 2 Abstract 1730

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