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How to measure miRNA expression - University of Bristol

How to measure miRNA expression Matt Barter Expansion of microRNA research 6000. 5000. Pubmed publications 4000. 3000 *. 2000. 1000. 0. 2005. 2000. 2001. 2002. 2003. 2004. 2006. 2007. 2008. 2009. 2010. 2011. 2012. Year Micro-managers of gene expression '. Talk plan 1. microRNAs Biogenesis Action Targetting 2. microRNA function Role of microRNAs in homeostasis, cell processes and disease Serum biomarkers Therapeutic microRNA inhibition 3. Measuring microRNA expression Extraction Assays and profiling Analysis 4. Experimental follow up 1. What are microRNAs? MicroRNAs ~22nt long post-transcriptional regulators of gene expression Small, single stranded non-coding RNAs Evolutionary ancient component of genetic regulation Current estimates ~1000 in humans - miRBase repository of sequence and nomenclature microRNA families similar sequence Clusters and multiple copies in genome Own promoter or intronic microRNA processing Wienholds 2005 FEBS letters What do microRNAs do?

E.g. microRNAs and disease In human cancer, miRNA expression profiles differ between normal tissues and the tumours that are derived from them, and differ between tumour types

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  Measure, Expression, Marin, How to measure mirna expression

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