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Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction , 12/2004 1 Laboratory for Environmental Pathogens Research Department of Environmental Sciences University of Toledo Polymerase Chain Reaction (PCR) Background information The Polymerase Chain Reaction (PCR) is an enzymatic process that allows for the detection of specific genes within an environmental DNA sample. PCR utilizes short, user defined DNA sequences called oligonucleotide primers, the sequence of which are complementary to target regions of genes known to encode for specific microbial functions ( contaminant degradation).

Polymerase Chain Reaction, 12/2004 5 MgCl 2 The concentration of MgCl 2 influences the stringency of the interaction between the primers and the template DNA. The range of MgCl 2 usually tested is from 0.5 - 4 mM in 0.5 mM increments, while …

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