Transcription of Real-time PCR handbook - Gene-Quantification
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Real-time PCR handbook - Gene-Quantification
Real-time PCR handbookThe image on this cover is of an OpenArray plate which is primarily used for mid-density Real-time PCR on the QuantStudio 12K Flex system. The figure above shows the commonly used formats for Real-time assays96- and 384-well plates384-well TaqMan Array cardsOpenArray plates162345 Basics of Real-time PCRD igital PCRE xperimental designPlate preparationData analysisTroubleshootingContentsBasics of Real-time Basics of Real-time PCR1 Introduction 3 Overview of Real-time PCR 4 Overview of Real-time PCR and Real-time PCR components 5 Real-time PCR analysis terminology 7 Real-time PCR fluorescence detection systems 11 Melting curve analysis 15 Use of passive reference dyes 16 Contamination prevention 17
DNA polymerase is active during reaction setup and the initial DNA denaturation step. Reverse transcriptase The reverse transcriptase (RT) is as critical to the success of qRT-PCR as the DNA polymerase. It is important to choose an RT that not only provides high yields of full-length cDNA, but also has good activity at high temperatures.
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