Transcription of Quantification strategies in real-time PCR Michael …
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A-Z of quantitative PCR (Editor: SA Bustin) Chapter3. Quantification strategies in real-time PCR 87 Quantification strategies in real-time PCR Michael W. Pfaffl Chaper 3 pages 87 - 112 in: A-Z of quantitative PCR (Editor: Bustin) International University Line (IUL) La Jolla, CA, USA publication year 2004 Physiology - Weihenstephan, Technical University of Munich, Center of Life and Food Science Weihenstephan, Freising, Germany Content of Chapter 3: Quantification strategies in real-time PCR Abstract 88 Introduction 88 Markers of a Successful real-time RT-PCR Assay 88 RNA Extraction 88 Reverse Transcription 90 Comparison of qRT-PCR with Classical End-Point Detection Method 91 Chemistry Developments for real-time RT-PCR 92 real-time RT-PCR Platforms 92 Quantification strategies in Kinetic RT-PCR 92 Absolute Quantification 93 Relative Quantification
A-Z of quantitative PCR (Editor: SA Bustin) Chapter3. Quantification strategies in real-time PCR 89 assessment30 of the starting RNA sample is particularly important for absolute quantification methods that normalize specific mRNA expression levels against total RNA ("molecules/g
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