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Taking qPCR to a higher level: Analysis of CNV …
Taking qpcr to a higher level: Analysis of CNV reveals the power ofhigh throughputqPCR to enhance quantitative resolutionSuzanne Weaver, Simant Dube, Alain Mir, Jian Qin, Gang Sun, Ramesh Ramakrishnan, Robert C. Jones,Kenneth J. Livak*Fluidigm Corporation, 7000 Shoreline Court, Suite 100, South San Francisco, CA 94080, USAarticle infoArticle history:Accepted 11 January 2010 Available online 15 January 2010 Keywords:qPCRReal-time PCRD igital PCRCopy number variationCNVM icrofluidic arrayHigh throughput qPCRabstractThis paper assesses the quantitative resolution of qpcr using copy number variation (CNV) as a error model is developed for real-time qpcr data showing how the precision of CNV determinationvaries with the number of replicates. Using samples with varying numbers ofXchromosomes, experi-mental data demonstrates that real-time qpcr can readily distinguish four copes from five copies, whichcorresponds to a difference in relative quantity. Digital PCR is considered as an alternative formof qpcr .
Taking qPCR to ahigher level: Analysis of CNV reveals the power ofhigh throughput qPCR to enhance quantitative resolution Suzanne Weaver, Simant Dube, Alain Mir, Jian Qin, Gang Sun, Ramesh Ramakrishnan, Robert C. Jones,
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