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10/12/20171 Basic MicrobiologyAssociate Director of Clinical Microbiology, UK HealthCareAssistant Professor, Department of Pathology and Laboratory MedicineUniversity of KentuckyDirector, Microbiology; Environmental Microbiology and PreparednessAssociate Director, Division of Laboratory ServicesKentucky Department for Public HealthVaneet Arora, MD MPH D(ABMM) FCCMO bjectives Identify normal flora, opportunistic and pathogenic organisms by body site Recognize empiric antimicrobial therapies based on microbe and body siteInfectious DiseaseInfluencing factors-How to decide empiric therapyAntimicrobial Patient related Age Adult vs Pediatric Body site Normally sterile Normal Commensals Immune status Immunocompetent Immunocompromised History Co-morbidities/ Family history Allergies Hepatic/Renal dysfunctionEnvironment related Setting Community vs Healthcare facility vs Long term care facility Outpatient vs Inpatient vs ICU Circulating organisms/strains Antibiograms Exposures Childcare/workplace Catheter/IV line/ PICC line/prosthesis TravelOrganism related Type Bacteria Fungi Parasites (Protozoa/Helminths)
10/12/2017 3 Gram staining Bacterial cell wall •Gram positive –Cocci •Staphylococcus –S. aureus » MSSA/MRSA –Coagulase negative staphylococci (CoNS) •Streptococcus –GAS/GBS
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