Transcription of Practical issues in FTIR spectroscopy Lab/demo section ...
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T BioMolecular Vibrational spectroscopy : Part 1: Principles of Infrared, raman Spectra and Techniques Tim Keiderling University of Illinois at Chicago Practical issues in FTIR spectroscopy Lab/demo section -Warwick CD Workshop T FTIR optical layout static polarization Polarizer (rotate , - typically wire grid) Sample Interfer- ometer Detector Source Digilab schematic Moving mirror Fixed mirror HeNe laser alignment Voice coil Air Bearing Aperture T Alternate, simpler FT-IR : Bomem Michelson Design often found in routine instruments, no air needed, highly stable T Acquisition of an Infrared Spectrum -1 0 1 2 4000 3980 3960 3940 3920 0 10 20 30 40 4000 3000 2000 1000 -2 -1 0 1 2 3 4000 3980 3960 3940 3920 1
Advantages of Raman Spectroscopy--Comparison Non-destructive Flexible sampling - any phase or size - no preparation 1µm sample area - Raman microscopy possible Glass cells - good medium for cell design, low cost Fiber optics - up to 100m, routine Water - weak scatterer - excellent solvent
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