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Practical issues in FTIR spectroscopy Lab/demo section ...

T BioMolecular Vibrational spectroscopy : Part 1: Principles of Infrared, Raman Spectra and Techniques Tim Keiderling University of Illinois at Chicago Practical issues in FTIR spectroscopy Lab/demo section -Warwick CD Workshop T FTIR optical layout static polarization Polarizer (rotate , - typically wire grid) Sample Interfer- ometer Detector Source Digilab schematic Moving mirror Fixed mirror HeNe laser alignment Voice coil Air Bearing Aperture T Alternate, simpler FT-IR : Bomem Michelson Design often found in routine instruments, no air needed, highly stable T Acquisition of an Infrared Spectrum -1 0 1 2 4000 3980 3960 3940 3920 0 10 20 30 40 4000 3000 2000 1000 -2 -1 0 1 2 3 4000 3980 3960 3940 3920 10 20 30 40 50 4000 3000 2000 1000 Interferogram of the source & sample Interferogram of the source Spectrum of the source (blank) Spectrum of the source & sample FT FT Fast Fourier transform done in computer Convert Interferogram to Spectrum (single beam) T Acquisition of an Infrared Spectrum 0 10 20 30 40 4000 3000 2000 1000 10 20 30 40 50 4000 3000 2000 1000 40 0 20 60 80 100 4000 3000 2000 1000 transmittance spectrum of the sample ( polystyrene film ) Spectrum of source only (no sample present) Spectrum of source & sample Divide by

Flexible sampling - any phase or size - no preparation 1µm sample area - Raman microscopy possible Glass cells - good medium for cell design, low cost Fiber optics - up to 100m, routine Water - weak scatterer - excellent solvent Enhanced by resonance, surface interactions

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