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Principles of MALDI-TOF mass spectrometry

Purdue-UAB Botanicals Center for Age-Related DiseasePrinciples of MALDIP rinciples of MALDI--TOF TOF mass spectrometrymass spectrometryStephen Barnes, Botanical Center Workshop 2002 Mass spectrometry Methods in Botanicals ResearchShining the light on samplesShining the light on samplesPurdue-UAB Botanicals Center for Age-Related Disease A focused laser beam, either in the UV or infra-red ranges, can evaporate compounds from the solid phase The resulting ions are injected into a tube (1 -2 m in length), accelerated and allowed to drift towards a detector. Their time-of-flightis proportional to their (MW)1/2 Matrix-Assisted Laser Desorption Ionization (MALDI)Accelerating pulsedetectorShort laserpulseFlight tube and drift region to measure the time-of-flight (TOF) MALDI-TOF ionizationN2laser pulse (337 nm)[M+H]+[M-H]-[M-H]-matrix ionsProtein/peptide/nucleotide/saccharid edeposited on crystal surfaceHow a TOF instrum

Desalting Ziptip. 684.11 78 9.5 7 1048.72 1122.79 1165.80 1277.83 1461.00 1705.16 1838.26 1937.22 1994.24 2137.21 2284.25 2384.27 26 47.50 0 5000 10000. Counts. 15000 1000 1500 2000 2500 3000 . Mass (m/z) Applications of time-of-flight. Purdue-UAB Botanicals Center for Age-Related Disease ...

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