Desalting
Found 6 free book(s)DNA Clean & Concentrator®-5 - Zymo Research
files.zymoresearch.comMar 17, 2021 · Efficient desalting of DNA with the removal of DNA polymerases, primers, and free dNTPs. DNA Clean-up From Enzymatic Reactions Efficient desalting of DNA with the removal of modifying enzymes, RNA polymerases, ligases, kinases, nucleases, phosphatases, endonucleases, etc. Post-Reverse Transcription (RT) & cDNA Clean-up
QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I)
schnablelab.plantgenomics.iastate.eduApr 01, 2007 · ordered from IDT. Primer purification: standard desalting. • SuperScript First-Strand Synthesis System for RT (Invitrogen). • SYBR Green PCR master mix (Applied Biosystems, cat log: 4309155). • 96-well plates (Stratagene, cat.: 410021) • 8-tube strip tubes (Stratagene, cat.: 410022) • Optical strip caps (Stratagene, cat.: 410024)
DNA EXTRACTION METHODS - Open University of Sri Lanka
www.ou.ac.lk•Desalting and buffer exchange •Removal of primers, linkers and Dr.L.Yatawaraunincorporated label. Concentrating DNA Microcon®100 Centrifugal Filter Unit •Patented deadstop allows for reliable and reproducible concentrate volumes •Inverted spin method of concentrate
P rotein preparation handbook - Thermo Fisher Scientific
assets.thermofisher.comZeba™ desalting products contain a unique resin and are specifically designed to provide consistent performance over a wide range of protein concentrations and sample sizes. High recovery of protein can be achieved even for dilute protein samples. • Concentration Protein concentration and diafiltration, similar to
The all-in-one” system for the inducible expression of shRNA
media.addgene.orgOn cohesive ends. Standard desalting is sufficient for oligo purification. Oligo annealing and ligation into pLKO-Tet-On: 1. Bring oligo powders to room temp and spin down for a few seconds. 2. Reconstitute oligos in ddH 2O to 0.1 nmol/µl : look at the amount of oligo printed on the tube and add the appropriate amount of ddH 2O (e.g. 21 nmol ...
Affinity Chromatography Principles and Methods Handbook
b3p.it.helsinki.fiGST Gene Fusion System Handbook 18-1157-58 Hydrophobic Interaction and Reversed Phase Chromatography Principles and Methods 11-0012-69 2-D Electrophoresis