Transcription of Propidium Iodide Nucleic Acid Stain - Thermo Fisher Scientific
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Propidium Iodide Nucleic Acid Stain - Thermo Fisher Scientific
Revised: 16 March 2006 | MP 01304 Table 1. Contents and storage Iodide solid (including FluoroPure PI *) (P1304MP, P21493)100 mgRoom temperatureProtect from light When stored as directed, product is stable at least 1 Iodide solution (P3566)1 mg/mL in water2 6 CProtect from light When stored as directed, solution is stable at least 6 months.* 98% fluorescence excitation/emission maxima: 535/617 nm, bound to Nucleic acidsPropidium Iodide Nucleic Acid StainIntroductionPropidium Iodide (PI) binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4 5 base pairs of PI also binds to RNA, necessitating treatment with nucleases to distinguish between RNA and DNA staining. Once the dye is bound to Nucleic acids, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ~30 40 nm to the red and the fluorescence emis-sion maximum is shifted ~15 nm to the Although its molar absorptivity (extinction coefficient) is relatively low, PI exhibits a sufficiently large Stokes shift to allow simultaneous detection of nuclear DNA and fluorescein-labeled antibodies, provided the proper optical filters are used.
Approximate fluorescence excitation/emission maxima: 535/617 nm, bound to nucleic acids Propidium Iodide Nucleic Acid Stain Introduction Propidium iodide (PI) binds to DNA by intercalating between the bases with little or no sequence preference and with a stoichiometry of one dye per 4–5 base pairs of DNA.1 PI also
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