Transcription of Protein Engineering Methods and Applications - IntechOpen
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Protein Engineering Methods and Applications - IntechOpen
2 Protein Engineering Methods and Applications Burcu Turanli-Yildiz1,2, Ceren Alkim1,2 and Z. Petek Cakar1,2, 1 Istanbul Technical University (ITU), Dept. of Molecular Biology and Genetics, 2 ITU Dr. Orhan Ocalgiray Molecular Biology, Biotechnology and Genetics Research Center (ITU-MOBGAM), Istanbul, Turkey 1. Introduction Protein Engineering is the design of new enzymes or proteins with new or desirable functions. It is based on the use of recombinant DNA technology to change amino acid sequences. The first papers on Protein Engineering date back to early 1980ies: in a review by Ulmer (1983), the prospects for Protein Engineering , such as X-ray crystallography, chemical DNA synthesis , computer modelling of Protein structure and folding were discussed and the combination of crystal structure and Protein chemistry information with artificial gene synthesis was emphasized as a powerful approach to obtain proteins with desirable
peptides upon design and synthesis of peptide analogs that are metabolically stable. Peptidomimetics is an importan t approach for bioorganic and me dical chemistry. It includes a variety of synthesis methods such as the use of a common intermediate, solid phase synthesis and combinatorial approaches (Venkatesan & Kim, 2002). www.intechopen.com
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