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Protein Reduction, Alkylation, Digestion

UWPR University of Washington Proteomics Resource Protein reduction , alkylation , Digestion Last updated 10/4/2011 Page 1 Protein reduction , alkylation , Digestion Contents Protein reduction , alkylation , Digestion .. 1 reduction / alkylation (DTT, IAA and UREA), Trypsin Digestion .. 2 Reagents and Materials (see Table 1) .. 2 reduction / alkylation (DTT, IAA and UREA) .. 2 Trypsin Digestion : .. 2 reduction / alkylation (DTT, IAA and RapiGest or PPS), Trypsin Digestion .

Oct 04, 2011 · Protein Reduction, Alkylation, Digestion Last updated 10/4/2011 Page 3 Reduction / Alkylation (DTT, IAA and RapiGest or PPS), Trypsin digestion: Works well for complex mixtures to be analyzed by mass spec. Reagents and Materials (see Table 1) Rapi. Gest SF Powder (Waters Corporation part # 1860018605 Pack of 1 ml Vials,) OR

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  Reduction, Protein, Digestion, Alkylation, Protein reduction

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