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Protocol for Nextera™ DNA Sample Prep Kit (Illumina ...

Continued Lit. #307 Nextera DNA Sample Prep Kit ( illumina -compatible) Cat. Nos. GA09115, GA091120, GA0911-50, GA0911-96, and GABC0950 The Nextera DNA Sample Prep Kit is designed to prepare genomic DNA libraries compatible with the illumina Genome Analyzer I and II and Hi-Seq 2000 sequencers. Nextera technology employs in vitro transposition to simultaneously fragment and tag DNA in a single-tube reaction, and prepare sequencer-ready libraries in under 2 hours. The Nextera library preparation procedure is a significant improvement upon current proce-dures, which generally consist of distinct DNA fragmentation, end-polishing, and adaptor-ligation steps. The Nextera library preparation procedure combines these steps into one (tagmentation), uses only 50 ng of starting DNA, and allows incor-poration of platform-specific tags and optional bar-codes. Covered by patents issued and pending.

Sequencing in the Same Channel: The Nextera sequencing primers are compatible with the Illumina sequencing primers, and can be used together. 9. Nextera PCR Enzyme: Use only Nextera PCR Enzyme for limited-cycle PCR (Step B, page 7). Other PCR systems have been tested and do not perform as well.

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