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AnintroductiontoNext-Generation Sequencing Technology

An introduction to Next-Generation Sequencing Technology For Research Use Only. Not for use in diagnostic procedures. Table of Contents Table of Contents 2. I. Welcome to Next-Generation Sequencing 3. a. The Evolution of Genomic Science 3. b. The Basics of NGS Chemistry 4. c. Advances in Sequencing Technology 5. Paired-End Sequencing 5. Tunable Coverage and Unlimited Dynamic Range 6. Advances in Library Preparation 6. Multiplexing 7. Flexible, Scalable Instrumentation 7. II. NGS Methods 8. a. Genomics 8. Whole-Genome Sequencing 8. Exome Sequencing 8. De novo Sequencing 9. Targeted Sequencing 9. b. Transcriptomics 11. Total RNA and mRNA Sequencing 11. Targeted RNA Sequencing 11. Small RNA and Noncoding RNAS equencing 11. c. Epigenomics 12. Methylation Sequencing 12. ChIP Sequencing 12. Ribosome Profiling 12. III. Illumina DNA-to-Data NGS Solutions 13. a. The Illumina NGS Workflow 13. b. Integrated Data Analysis 13. IV. Glossary 14. V. References 15. For Research Use Only.

Figure 4:Paired-EndSequencingandAlignment—Paired-endsequencingenablesbothendsoftheDNAfragmenttobesequenced.Becausethedistancebetweeneach pairedreadisknown ...

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