PURE CULTURE TECHNIQUES I. OBJECTIVES

Microbiology BIOL 275 Dr. Eby Bassiri 1 pure CULTURE TECHNIQUES I. OBJECTIVES To demonstrate good aseptic technique in CULTURE transfer or inoculation and in handling sterile materials. To demonstrate skill in isolation of organisms from a mixed CULTURE using selective and differential media. To isolate microorganisms from a wide variety of sources and describe their colonial morphology. II. INTRODUCTION Most environments carry a mixed microbial population. To fully appreciate the contribution of each group of organisms to the ecology of the mass, one must first dissect this mixed CULTURE to obtain single colonies. The single colony is transferred (picked) to a fresh medium to obtain a larger, homogeneous CULTURE that may be studied and characterized by a variety of TECHNIQUES .

Aseptic techniques (also called sterile techniques) are defined as the processes ... will save yourself a lot of time and energy and avoid erroneous results if a few simple ... maintenance media used for many environmental organisms that do not have special growth requirements, such as E. coli, ...

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