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qPCR Quantification Protocol Guide - Boston University

ILLUMINA PROPRIETARYC atalog # SY-930-1010 Part # 11322363 Rev. ASeptember 2009qPCR Quantification Protocol GuideFOR RESEARCH USE ONLYTo p i c s3 Introduction5 User-Supplied Consumables and Equipment7 Select Control Template8 Dilute qpcr Control Template9 Dilute Libraries10 Prepare Reaction Mix11 Aliquot to 96-Well Plate12 Quantify by qPCR13 Analyze15 Appendix A - Determine Cluster Numbers for Control Library17 Appendix B - Sample Preparation for Cluster Generation19 Appendix C - Determine Relative GC Content of LibraryThis document and its contents are proprietary to Illumina, Inc. and its affiliates ( Illumina ), and are intended solely for the contractual use of its customers and for no other purpose than to use the product described herein.

3 qPCR Quantification Protocol Guide Introduction This document describes a qPCR method for quantifying libraries generated using the Illumina® sample preparation protocols. qPCR is a method of quantifying DNA based on PCR. qPCR tracks target concentration as a

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