Transcription of Rationale for Using Direct Bone Marrow Aspirate as a ...
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Rationale for Using Direct Bone Marrow Aspirate as a ...
Send Orders for Reprints to The Open Open Stem Cell Journal, 2013, 4, 7-14 7 1876-8938/13 2013 Bentham Open Open Access Rationale for Using Direct bone Marrow Aspirate as a Proliferant for Re-generative Injection Therapy (Prolotherapy) R. A. Hauser1,* and E. Eteshola2 1 Caring Medical and Rehabilitation Services, 715 Lake St., Oak Park IL 60301 USA 2536 Whitson Drive, Gahanna, OH 43230, USA Abstract: Adult mesenchymal stem cells (MSCs) obtainable from autologous bone Marrow aspirates have generated tre-mendous interest in the medical and scientific communities in the last two decades and are currently being investigated by a of interested physicians for use in point-of-care stem cell therapies due to their great potential to differentiate into multi-ple cell lineages such as bone , cartilage, muscle, tendon, and nerve. However, as these stem cells are found in very low numbers in adult tissue, centrifugal concentration or expansion through in vitro culturing has been pursued to obtain higher numbers of efficacious regenerative therapeutic applications.
in vitro culturing; moreover, currently used centrifugal concentration techniques do not significantly concentrate MSCs from bone marrow aspirate, thus, defeating the purpose of this manipulative step. On the other hand, preliminary results
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