Real-time PCR handbook

Real-time PCR handbookSingle-tube assays96- and 384-well plates384-well TaqMan Array CardsOpenArray platesCommonly used formats for Real-time Basics of Real-time PCR Digital PCR Experimental design Plate preparation Data analysis TroubleshootingBasics of Real-time introduction Overview of Real-time PCR Overview of Real-time 4 PCR Real-time PCR analysis technology Real-time PCR fluorescence 10 detection Melting curve analysis Passive reference dyes Contamination prevention Multiplex Real-time PCR Internal controls and 18 reference Real-time PCR 19 instrument calibration1 Basics of Real-time PCR IntroductionThe polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology. Using PCR, specifi c sequences within a DNA or complementary DNA (cDNA) template can be copied, or amplifi ed, many thousand- to million-fold using sequence-specifi c oligonucleotides, heat-stable DNA polymerase , and thermal cycling.

1.1 Introduction The polymerase chain reaction (PCR) is one of the most powerful technologies in molecular biology. Using PCR, specifi c sequences within a DNA or complementary DNA (cDNA) template can be copied, or “amplifi ed,” many thousand- to million-fold using sequence-specifi c oligonucleotides, heat-stable DNA polymerase,

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  Introduction, Chain, Reactions, Polymerase chain reaction, Polymerase

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