Transcription of Red Blood Cell Lysis Protocols J 20110916
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Red Blood Cell Lysis Protocols J 20110916
Introduction:Red Blood Cell (RBC) Lysis Buffer (Cat. No. 420301) RBC Lysis Buffer Method I: 1. 2. 350xg 3. 10 RBC Lysis Buffer 1 RBC Lysis Buffer 3 5ml 4. 4 5 5. 1 PBS 10 20ml 6. 350xg BioLegend Cell Staining Buffer Cat. No. 420201 7. Method II: 1. 10 RBC Lysis Buffer 2. 100 l 1 RBC Lysis Buffer 2ml 3. 1 RBC Lysis Buffer 10 15 4. 350xg BioLegend Cell Staining Buffer Cat. No. 420201 References:1. Muirhead K., et al. 1986. Ann. NY Acad. Sci. 468 Mishell, B., et al. 1980. Preparation of Mouse Cell Suspensions. In: Selected Methods in Cellular Immunology. Freeman & Company, San Francisco, p. List:1.
Introduction: Red Blood Cell (RBC) LysisBuffer (Cat. No. 420301)は、末梢血液由来の赤血球と同様に、脾臓等の造 血組織の細胞懸濁液において赤血球の溶解に対して最適化されてい …
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