RNA integrity and the effect on the ... - Gene …

ReviewRNA integrity and the effect on the real-timeqRT-PCR performanceSimone Fleigea, Michael W. Pfaffla,b,*aPhysiology Weihenstephan, Center of Life and Food Sciences (ZIEL),Technical University of Munich, 85350 Freising, GermanybTATAA Biocenter Germany, Freising-Weihenstephan, GermanyAbstractThe assessment of RNA integrity is a critical first step in obtaining meaningful gene expres-sion data. Working with low-quality RNA may strongly compromise the experimental resultsof downstream applications which are often labour-intensive, time-consuming, and highlyexpensive. Using intact RNA is a key element for the successful application of modern mole-cular biological methods, like qRT-PCR or micro-array analysis. To verify RNA quality now-adays commercially available automated capillary-electrophoresis systems are available whichare on the way to become the standard in RNA quality assessment.

Review RNA integrity and the effect on the real-time qRT-PCR performance Simone Fleige a, Michael W. Pfaffl a,b,* a Physiology Weihenstephan, Center of Life and Food Sciences (ZIEL), Technical University of Munich, 85350 Freising, Germany b TATAA Biocenter Germany, Freising-Weihenstephan, Germany Abstract

Tags:

  Gene, Integrity, Rna integrity and the effect on, Effect

Information

Related search queries