Transcription of RNA isolation - Macherey-Nagel AG
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RNA isolation - Macherey-Nagel AG
macherey -NAGELEN ISO 9001EN ISO 13485 CERTIFIEDMACHEREY-NAGEL GmbH & Co. KG Neumann-Neander-Str. 6 8 52355 D ren GermanyFrance: Macherey-Nagel EURLTel.: +33 388 68 22 68E-mail: AGTel.: +41 62 388 55 00E-mail: international:Tel.: +49 24 21 969-0E-mail: : +1 484 821 0984E-mail: isolationUser manualNucleoSpin RNA XSSeptember 2015 / Rev. 09 Macherey-Nagel GmbH & Co. KG Neumann-Neander-Str. 6 8 52355 D ren Germany Tel.: +49 24 21 969-270 Fax: +49 24 21 969-199 isolationProtocol-at-a-glance (Rev. 09)XSNucleoSpin RNA XS1 Supply sampleUse up to 105 cultured cells or 5 mg tissue samples2 Lyse and homogenize cells100 L RA1 2 L TCEPMix3 Add Carrier RNA5 L Carrier RNA working solutionMix4 Filtrate lysate (optional)11,000 x g, 30 s5 Adjust RNA binding condition100 L 70 % ethanolMix6 Bind RNALoad lysate11,000 x g, 30 s7 Desalt silica membrane100 L MDB11,000 x g, 30 s8 Digest DNA25 L DNase reaction mixtu
MACHEREY-NAGEL – 09 / 2015, Rev. 09 3 RNA isolation Table of contents 1 Components 4 1.1 Kit contents 4 1.2 Reagents, consumables, and equipment to be supplied by user 5
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