Transcription of RNA isolation - Macherey-Nagel AG
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RNA isolation - Macherey-Nagel AG
MACHEREY-NAGELEN ISO 9001EN ISO 13485 CERTIFIEDMACHEREY-NAGEL GmbH & Co. KG Neumann-Neander-Str. 6 8 52355 D ren GermanyFrance: Macherey-Nagel EURLTel.: +33 388 68 22 68E-mail: AGTel.: +41 62 388 55 00E-mail: international:Tel.: +49 24 21 969-0E-mail: : +1 484 821 0984E-mail: RNA isolationUser manualNucleoSpin RNA PlusJune 2016 / Rev. 03A046994 / 0762 RNA isolationProtocol-at-a-glance (Rev. 03) Macherey-Nagel GmbH & Co. KG Neumann-Neander-Str. 6 8 52355 D ren Germany Tel.: +49 24 21 969-270 Fax: +49 24 21 969-199 RNA Plus1 Homogenize sample and lyse sample350 L LBP2 Remove gDNA and filtrate lysate11,000 x g, 30 s3 Adjust RNA binding conditions100 L BSMix4 Bind RNALoad sample11,000 x g, 15 s5 Wash and dry silica membrane1st wash2nd wash3rd wash200 L WB1600 L WB2250 L WB21st and 2nd 11,000 x g, 15 s3rd11,000 x g, 2 min6 Elute RNA30 L RNase-free H2O11,000 x g, 1 min30 L RNase-free H2O11,000 x g, 1 min3 RNA isolationMACHEREY-NAGEL 06/ 2016, Rev.
3 RNA MACHEREY-NAGEL – 06/ 2016, Rev. 03 Table of contents 1 Components 4 1.1 Kit contents 4 1.2 Reagents, consumables, and equipment to be supplied by user 5
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