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RNeasy MiniHandbook

RNeas y Min i Ha nd bookRNeasy Mini KitForpurificationof totalRNA fro m animalcells,animal tissues, ba cteria,andye as t, and forRNAcleanupRNeasy Pr ote ct MiniKitForimmed iat e sta bilizationof RNAin har vestedanimal tissuesand subsequenttot al RNApurificationRNeasy Plant Mini KitForpurificationof totalRNA fro m plants andfilamentousfun giFo urt h Edit ionJune 2012 Sample& AssayTechnologiesQI AGENS ample and AssayTec hnologiesQIA GENis thele adingpr ovider of innovativesampleandassay technologies,enablingtheisolati onanddetectionofconte ,high-qual ityproduct s and servicesensuresuccessfromsa mple to EN set s sta ndards in: Puri fi ca tionof DNA, RNA,andpr oteins Nuclei c aci d andpr otein assays mi croRNAre searchandRNAi Aut omationof sample andassay tec hnologiesOur mi ss ionis to en able youto achieveoutstandingsu in for mation, nte ntsKit Con tent s4 Storage5In ten de d Use5 Safe ty Info rm ati on6 Qual ity Con tr ol6In troduction7 Princip le andpr oc edure8De sc ri pt ionof protocol s10 Equipm ent and Reagentsto Be Suppliedby Use r13Im portant No te s16De termining th e amountof sta rting mate rial16 Handli ngandstoring sta rt ingmater ia l18Di srupti

reagent preserves RNA for up to 1 day at 37°C, 7 days at room temperature (15–25°C), or 4 weeks at 2–8°C, allowing transportation, storage, and shipping of samples without ice or dry ice. Alternatively, the samples can be archived at –20°C or –80°C. During storage or transport in RNA later RNA Stabilization Reagent, even at

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