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Seahorse XF Cell Mito Stress Test Kit User Guide

Agilent TechnologiesAgilent Seahorse XF Cell Mito Stress Test KitUser Guide Kit 103015-100 Notices Agilent Technologies, Inc. 2019No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or transla-tion into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright Part Number103016-400 Kit Part Number103015-100 EditionSecond edition, May 2019 Revision G0 Printed in USAA gilent Technologies, Inc. 2850 Centerville Road Wilmington, DE 19808-1610 USAW arrantyThe material contained in this docu-ment is provided as is, and is sub-ject to being changed, without notice, in future editions. Further, to the max-imum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a par-ticular purpose.

Agilent Seahorse XF Cell Mito Stress Test Kit User Guide 7 Carbonyl cyanide-4 (trifluoromethoxy) phenylhydrazone (FCCP) is an uncoupling agent that collapses the proton gradient and disrupts the mitochondrial memb rane potential. It is the 2nd injection following Oligomycin. As a result, electron

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