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Separation by Chromatography Methods - Sinica

Separation by Chromatography Methods Analytical Biochemistry Principle of Separation techniques Methods Based on Polarity ( ). Biochemistry and Molecular Biology Partition Chromatography Ion Exchange Chromatography Gel Filtration Chromatography Affinity Chromatography 1. How Does Chromatography Work? Chromatography is a method for separating the components of a mixture by differential adsorption between a stationary phase and a mobile (moving) phase Mobile Phase ( ). Liquid Liquid Liquid Solid Partition Adsorption Stationary Phase Chromatography Chromatography ( ). 2. Principles of Separation Techniques AB Molecular Physical property Separation technique Characteristic Polarity Volatility Gas-liquid Chromatography Solubility Liquid-liquid Chromatography Adsorptivity Liquid-solid Chromatography Ionic Charge Ion-exchange Chromatography Electrophoresis Gel permeation Size ( mass ) Diffusion Chromatography Dialysis Shape Sedimentation Ultracentrifugation Liquid binding Affinity Chromatography 3.

3.1 Principle of Separation techniques 3.2 Methods Based on Polarity (3.2.1-3.2.3) Biochemistry and Molecular Biology ... MS – Mass Spectrometry – Mass to charge ratio (m/z) – Allows specific compound ID . 26 HPLC Diode Array Detection Analysis - Absorbance is measured at two or

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