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Seracare stability of genomic DNA at various storage ...

International Society for Biological and Environmental Repositories stability of genomic DNA at various storage Conditions (ISBER) 2009 Annual Meeting May 12-15, 2009 Poster#: QAC 03. Wu J, Cunanan J, Kim L, Kulatunga T, Huang C and Anekella B. Seracare Life Sciences, Milford, MA. INTRODUCTION RESULTS A Figure 3: stability of genomic DNA SUMMARY. M 1 2 3 4 5 6 7 8 9 M 1 2 3 4 5 6 7 8 9. Upon Multiple Freeze Thaw Cycles Advances in recombinant technology and completion of the Human genomic DNA aliquots stored at -20 C and -80 C were stable Genome Project paved the way for identification and detection of genetic Panel A: Agarose gel electrophoresis Table 1: Parameters to analyse the stability of genomic DNA and PCR amplification (536 bp) of for over 24 months (real time stability still ongoing) (Figure 1).

the extracted DNA. The presence of high molecular weight DNA with no smearing on the gel suggests that the DNA is of high quality. PCR amplification was performed on 50ng of purified DNA by using theβ-globin primer pair that amplifies a ~536 bp DNA fragment. Successful amplification suggests that the extracted DNA does not contain any

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