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Size Exclusion Chromatography

Size Exclusion Chromatography Principles and Methods18-1022-18 AL 11/2014GE and GE monogram are trademarks of General Electric Company. KTA, Biacore, BioProcess, HiLoad, HiPrep, HiScale, HiTrap, MabSelect , MidiTrap, MiniTrap, MultiTrap, Sephacryl, Sephadex, Sepharose, SpinTrap, Superdex, Superose, Tricorn, and UNICORN are trademarks of GE Healthcare Company or one of its subsidiaries. BCA is a trademark of Thermo Fisher Scientific LLC. Coomassie is a trademark of Imperial Chemical Industries Limited. Microsoft and Excel are registered trademarks of the Microsoft Corporation. Triton is a trademark of Union Carbide Chemicals and Plastic Company Inc. Tween is a trademark of Uniqema Americas other third-party trademarks are the property of their respective Sepharose products, Ni Sepharose products and Fe Sepharose products: These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.

Biomolecules are purified using chromatography techniques that separate them according to differences in their specific properties, as shown in Figure I.1. Property Technique Size Size exclusion chromatography (SEC), also called gel filtration (GF) Hydrophobicity Hydrophobic interaction chromatography (HIC) Reversed phase chromatography (RPC)

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