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Experiment 6 — Thin-Layer Chromatography

Chem 21 Fall 2009 1 Experiment 6 Thin-Layer Chromatography _____ Pre-lab preparation (1) An introduction to TLC can be found at (ignore the little green note blocks within that text, and we'll be using screw-cap jars, not beakers.) (2) In a few sentences, describe how TLC works, and sketch what a TLC plate might look like after development with solvent. (3) Use your drawing to show how the Rf value is determined. Thin-Layer Chromatography (TLC) is an extremely valuable analytical technique in the organic lab.

In column chromatography, the sample is carried down a column of silica or alumina by solvent, and the separate components of the mixture are captured as they elutes from (exit) the column. This can be done by allowing the solvent to flow …

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