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Available online Journal of Chemical and Pharmaceutical Research, 2014, 6(6):1695-1698 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 1695 Spectrophotometric method for the determination of sodium hyaluronate with basic bisphenylnaphthylmethane dyes Lianhui Chen1, Shaopu Liu2, Hongqun Luo2 and Xiaoli Hu2 1 Department of Chemistry, North-Sichuan Medical College, Nanchong 2 School of Chemistry and Chemical Engineering, Southwest-China University, Chongqing _____ ABSTRACT In weak acidic medium, bisphenylnaphthylmethane dyes, such as night blue (NB),Victoria blue B (VBB) and Victoria blue 4R (VB4R) , react rapidly with sodium hyaluronate to form complexes, which causes the change of absorption spectra, the decrease of absorbance near 612nm(NB),616nm(VBB),585nm(VB4R), separately, and A(=A-A0) values are proportional to the concentration of sodium hyaluronate, Beer s law is obeyed in the range between ~ mg/L(NB) , ~ (VBB) and ~ mg/L(VB4R)
Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2014, 6(6):1695-1698 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5
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