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Spectroscopy: Principles, Theory, Techniques and Applications

PDF generated using the open source mwlib toolkit. See for more generated at: Sat, 30 May 2009 17:33:36 UTCS pectroscopy: Principles, Theory, Techniques andApplicationsSpectroscopy2 spectroscopy -An IntroductionSpectroscopyAnimation of the dispersion of light as it travels through atriangular prismSpectroscopy was originally thestudy of the interaction betweenradiation and matter as a functionof wavelength ( ). In fact,historically [1], spectroscopyreferred to the use of visible lightdispersed according to itswavelength, by a prism. Laterthe concept was expanded greatlyto comprise any measurement of aquantity as function of eitherwavelength or frequency. Thus italso can refer to a response to analternating field or varyingfrequency ( ).

Fluorescence spectroscopy uses higher energy photons to excite a sample, which will then emit lower energy photons. This technique has become popular for its biochemical and medical applications, and can be used for confocal microscopy, fluorescence resonance energy transfer, and fluorescence lifetime imaging. X-ray When X-rays of sufficient

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  Energy, Spectroscopy, Resonance, Fluorescence, Fluorescence spectroscopy, Fluorescence resonance energy

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