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Technical BulletinStandardizing Application Setup Across Multiple Flow Cytometers Using BD FACSDiva Version 6 SoftwareBD BiosciencesMarch 2012 Technical BulletinStandardizing Application Setup Across Multiple Flow Cytometers Using BD FACSDiva Version 6 SoftwareEllen Meinelt, Mervi Reunanen, Mark Edinger, Maria Jaimes, Alan Stall, Dennis Sasaki, Joe Trotter Contents 1 Abstract 2 Introduction and Background 4 Part 1. Characterization of a Cy tometer 6 Part 2. Rules Used to Create Application Settings 7 Part 3. Creating the Application Settings 8 Part 4. Cytometer Standardization 11 Part 5. Verification of Standardization Accuracy 12 Appendix 16 AcknowledgmentsAbstractStandardizing Application Setup can ensure consistency of results over time Across Multiple systems. To achieve this consistency, each cytometer must be properly and efficiently characterized and its performance levels maintained. BD Cytometer Setup and Tracking (CS&T) software and BD Cytometer Setup and Tracking beads (dim, mid, and bright) were developed to deliver this characterization and performance maintenance capability.
Technical Bulletin Standardizing Application Setup Across Multiple Flow Cytometers Using BD FACSDiva™ Version 6 Software BD Biosciences March 2012
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