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Streptococcus Laboratory General Methods

1 The reference used for compiling the Methods in Section I is: Murray, , Baron, E. J., Jorgensen, , Pfaller, , and Yolken, Manual of Clinical Microbiology, 8th ed. ASM Press: Washington, DC, 2003. The Streptococcus species identification Methods in Section II were compiled by Dr. Lynn Shewmaker. Also thanks to input from several individuals, including Richard Facklam and Lucia Teixeira. Section I. 1. Accuprobe-Enterococcus Accuprobe-Pneumococcus Test .. formation in carbohydrate solubility Test ..98. CAMP Catalase Clindamycin Esculin 1312. Gas from MRS Growth at 10 & 1917. Lancefield Group amino peptidase (LAP)..2119. Litmus Milk NaCl Tolerace 2624. Pyridoxal Requirement Test (Vitamin B6)..2725. Pyrrolidonlarylamindase (PYR).

1.s decarboxylase broth containing argini Moeller’ ne. The medium is commercially available. 2. Pipet or loop . IV. Procedure . 1. Add 1-3 drops of culture suspension to the tube of Moeller's decarboxylase medium containing arginine . 2. Immediately overlay with sterile mineral oil (about 1 to 2 ml). 3. The medium is incubated at 35Ε C for ...

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