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Structure/function relationship in DNA-binding proteins

Structure/function relationship in DNA-binding proteins Devlin Chapter u General description of transcription factors (TFs) u Sequence-specific interactions between DNA and proteins u Structural motifs of TF DNA-binding domains (helix-turn-helix; zinc fingers; bZIP; helix-loop-helix; beta-scaffold) u DNA- protein interactions in transcription u RNA polymerase and preinitiation complex for transcription u Mechanism of gene activation by transcription factors PHRM 836 September 22, 2015 proteins are the processing units for transcription PHRM 836, 22 Sept 2015 2 protein -DNA interactions } DNA-binding proteins } Transcription factors } Polymerases & nucleases } Transcription factors utilize a wide range of DNA-binding structural motifs } Interaction between dimeric proteins and palindromic sequences are commo

Function=control of which gene gets transcribed. ! TATA box is in front of start site of transcription and TBP binding creates a marks for transcription start ! Binding induces significant changes in the DNA structure ! Enables good fit between the protein and DNA bases. ! The binding of TBP directs assembly of

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