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Structure/function relationship in DNA-binding proteins

Structure/function relationship in DNA-binding proteins Devlin Chapter u General description of transcription factors (TFs) u Sequence-specific interactions between DNA and proteins u Structural motifs of TF DNA-binding domains (helix-turn-helix; zinc fingers; bZIP; helix-loop-helix; beta-scaffold) u DNA- protein interactions in transcription u RNA polymerase and preinitiation complex for transcription u Mechanism of gene activation by transcription factors PHRM 836 September 22, 2015 proteins are the processing units for transcription PHRM 836, 22 Sept 2015 2 protein -DNA interactions } DNA-binding proteins } Transcription factors } Polymerases & nucleases } Transcription factors utilize a wide range of DNA-binding structural motifs } Interaction betw

DNA sequence-specificity of DNA- binding proteins ! Sequence-specific interactions ! Frequently involve DNA major groove ! Base-specific H-bond donor, acceptors, and nonpolar groups are recognized by DNA-binding proteins ! DNA structure can deviate from classic B-form helix, and therefore be specifically recognized by a protein. !

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