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Supercritical Fluid Chromatography

Supercritical Fluid Chromatography 1. What is Supercritical Fluid 2. Supercritical Fluid Extraction 3. Supercritical Fluid Chromatography (SFC). 4. Theory of SFC. 5. Instrumentation 6. Applications Supercritical Fluid Chromatography 1. What is Supercritical Fluid Supercritical Fluid is a state of matter that is intermediate between a gas and liquid in its properties. This state formed when a gas or liquid solvent is subjected to temperature and pressure condition exceeding a particular critical point. The temperature and pressure at which this pint Occurs are known as the Critical temperature and Critical pressure and are Characteristic of the solvent. Beyond this point, the solvent Will be neither a gas or liquid, but will possess properties of both phases . Whether this Supercritical Fluid acts more like a gas or liquid will depend on the pressure and temperature 2.

3. Supercritical fluid chromatography (SFC) a. SFC isa chromatographic technique in which the mobile phase is a supercritical fluid. b. The use of a supercritical fluid mobile phase in chromatography was first proposed in 1958 by J. Lovelock. The first actual report use of this in a chromatographic system was in 1962 by Klesper et al, who used it

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