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Thin Layer Chromatography (TLC) - Boston College

1 Experiment 4: Thin Layer Chromatography (TLC) In LTOC, read the introduction to Part 4, Chromatography , on pp 253-254, then read pp 255-269, Chapter 18. View video Thin- Layer Chromatography at Thin Layer Chromatography (TLC) is an easy, convenient and inexpensive way to determine how many components are in a mixture and, in many instances, can be used to identify the components as well. In today s experiments, you will gain experience with both paper and thin Layer Chromatography , you will work with a variety of developing solvents, and you will use several different techniques to visualize the spots of a chromatogram.

Paper chromatography, which will be used to separate amino acids, is a form of partition chromatography (see LTOC page 253). Water, a component of the developing solvent, forms hydrogen bonds with the fibers of the paper and serves as the stationary phase. The organic liquids that are also present in the developing solvent serve as the mobile ...

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