Transcription of SUPERCRITICAL FLUID CHROMATOGRAPHY - Agilent
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SUPERCRITICAL FLUID CHROMATOGRAPHY - Agilent
SUPERCRITICAL FLUID . CHROMATOGRAPHY . Primer SUPERCRITICAL FLUID . CHROMATOGRAPHY . Primer Terry A. Berger This information is subject to change without notice. Agilent Technologies, Inc., 2015. Printed in the USA, July 1, 2015. 5991-5509EN. CONTENTS. Contents III. Foreword VI. About the Author XI. Introduction XIII. Symbols XIV. Abbreviations XIV. 1 I ntroduction to SUPERCRITICAL FLUID CHROMATOGRAPHY 1. What is SFC? 1. Why deploy SFC? 2. What can SFC separate? 12. What's in a name? 16. 2 The Mobile Phase 19. Why use CO 2? 19. Using 100 % CO 2 21. Modifiers or cosolvents 23. Additives 31. Extending solute polarity with added water 34. 3 The Stationary Phase 35. Materials 35. Achiral bonded phases 35. Stationary phase comparisons 40. Relationship between particle diameter and column dimensions 41. Recommended column dimensions 45. Columns for chiral separations 48. III. 4 E ffect of Mobile Phase Variables on Retention and Selectivity 49.
published in such journals as Analytical Chemistry, and Science, as well as related application notes. These works resulted in a customer demand that led to the creation of a commercial kit that converted a Hewlett-Packard model HP 1084 HPLC instrument into an SFC system. Thus, commercial SFC was born.
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