Transcription of Supporting Information - PNAS
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Supporting Information - PNAS
Supporting Information Liu et al. SI Materials and Methods Transfection of MicroRNA Mimics and Inhibitors. Peritoneal macro- Reagents. Thioglycolate and LPS from Escherichia coli 0111:B4 phages were transfected with 40 nM control microRNA mimics were from Sigma-Aldrich. PAM3 CSK4 and poly(I:C) were from (Ambion) or mouse miR-147 mimics (Ambion) using HIPerFect Invivogene. Rabbit anti-p65 and rabbit anti-STAT1 antibodies transfection reagents (Qiagen) according to the manufacturer's were from Santa Cruz. Protein G agarose beads were from instructions. At 2 days after transfection, the cells were washed Pierce. three times and then underwent treatments. Similarly, cells were transfected with 40 nM control locked nucleic acid (LNA). Mice. C57BL/6 mice were purchased from NCI-Frederick. modified inhibitors (Exiqon) or mouse LNA miR-147 inhibitors TRIF / mice were purchased from Jackson Labs. MyD88 / (Exiqon).
Cytokine ELISA. Immunoreactive mouse TNF- and mouse IL-6 was quantified using commercially available ELISA kits (R&D Systems), …
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