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Test Method Protocol for Solubility Determination (2003)

Test Method Protocol for Solubility Determination Phase III - Validation Study September 24, 2003 TEST Method Protocol for Solubility Determination In Vitro Cytotoxicity Validation Study Phase III September 24, 2003 Prepared by The National Toxicology Program (NTP) Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM) Based on Standard Operating Procedure Recommendations from an International Workshop Organized by the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM) National Institute of Environmental Health Sciences (NIEHS) National Institutes of Health (NIH) Public Health Service Department of Health and Human Services 1 Test Method Protocol for Solubility Determination Phase III - Validation Study September 24, 2003 TEST Method Protocol Solubility Determi

The solubility test procedure is based on attempting to dissolve chemicals in various solvents with a increasingly rigorous mechanical techniques. The solvents to be used, in the order of preference, are cell culture media, DMSO, and ethanol. Solubility shall be determined in a step-wise procedure that

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