Transfection Methods Overview - Bio-Rad

Methods MethodsOverview2 OverviewCommon Transfection MethodsReagent-Based MethodsLipidsCalcium phosphateCationic polymersDEAE-dextranActivated dendrimersMagnetic beadsInstrument-Based MethodsElectroporationBiolistic technologyMicroinjectionLaserfection/opt oinjectionVirus-Based Methods41 Terminology2 Factors Affecting TransfectionHost CellCell healthCell cultureGenetic MaterialDNA quality and quantityTransfection : Introduction of foreign DNA into the nucleus of eukaryotic cells . cells that have incorporated the foreign DNA are called transfectants: cells that have integrated foreign DNA in their transfectants: Foreign DNA does not integrate in the genome but genes are expressed for a limited time (24 96 hours).TerminologyTransfection MethodsTerminology4 Transfection Affecting Transfection5 Host CellCell healthCell cultureGenetic MaterialDNA quality and quantityFactors Affecting Transfection2 Transfection CellCell Health cells should be grown in appropriate medium with all necessary factors Cultures must be free of contamination Fresh medium must be used if it contains chemically unstable components, such as thiamine cells should be incubated at 37 C with CO2 supplied at the correct percentage (5 10%) and 100% relative humidity cells should be maintained in log phase growthCell HealthFactors Affecting Transfection6 Transfection CultureConfluency and Growth Phase cells should be transfected at 40 80% confluency (cell type dependent)

Transfection Methods www.bio-rad.com/transfection Factors Affecting Transfection 5 Host Cell Cell health Cell culture Genetic Material DNA quality and quantity

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