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Transfection Methods Overview - Bio-Rad

Methods MethodsOverview2 OverviewCommon Transfection MethodsReagent-Based MethodsLipidsCalcium phosphateCationic polymersDEAE-dextranActivated dendrimersMagnetic beadsInstrument-Based MethodsElectroporationBiolistic technologyMicroinjectionLaserfection/opt oinjectionVirus-Based Methods41 Terminology2 Factors Affecting TransfectionHost CellCell healthCell cultureGenetic MaterialDNA quality and quantityTransfection : Introduction of foreign DNA into the nucleus of eukaryotic cells. Cells that have incorporated the foreign DNA are called transfectants: Cells that have integrated foreign DNA in their transfectants: Foreign DNA does not integrate in the genome but genes are expressed for a limited time (24 96 hours).

Cationic Lipids: Liposomes/Lipoplexes Cationic lipids are amphiphilic molecules that have a positively charged polar head group linked, via an anchor, to an apolar hydrophobic domain generally comprising two alkyl chains Structural variations in the hydrophobic domain of cationic lipids include the length and the degree of

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