Transcription of TRIzol Reagent Product description
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TRIzol Reagent Product description
TRIzol ReagentCatalog Numbers 15596026 and 15596018 Doc. Part No. Pub. No. MAN0001271 Rev. ! Read the Safety Data Sheets (SDSs) and follow thehandling instructions. Wear appropriate protective eyewear,clothing, and gloves. Safety Data Sheets (SDSs) are availablefrom descriptionInvitrogen TRIzol Reagent is a ready-to-use Reagent , designed toisolate high quality total RNA (as well as DNA and proteins) from celland tissue samples of human, animal, plant, yeast, or bacterial origin,within one hour. TRIzol Reagent is a monophasic solution of phenol,guanidine isothiocyanate, and other proprietary components whichfacilitate the isolation of a variety of RNA species of large or smallmolecular size. TRIzol Reagent maintains the integrity of the RNAdue to highly effective inhibition of RNase activity while disruptingcells and dissolving cell components during sample Reagent allows for simultaneous processing of a large numberof samples, and is an improvement to the single-step RNA isolationmethod developed by Chomcynski and Sacchi (Chomczynski andSacchi, 1987).
Note: The glycogen is co-precipitated with the RNA, but does not interfere with subsequent applications. b. Add 0.5 mL of isopropanol to the aqueous phase, per 1 mL of TRIzol™ Reagent used for lysis. c. Incubate for 10 minutes at 4°C. Total RNA precipitate forms a white gel-like pellet at the bottom of the tube. e.
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